PD Training

1. BIOTECHNOLOGY  COMPONENT (PD DANG THUY BINH)

Project title: Effect of climate change on population dynamics of the swimming crab, Portunus pelagicus, and the epidemiology of selected disease causing organisms by PD Dang Thuy Binh

Recent obtained results

Sampling

crab, Portunus pelagicus was collected at Nha Trang Bay, O Loan Bay, and Song Cau (Khanh Hoa, Phu Yen, and Binh Dinh Province,) and Phu Quoc (Kien Giang Province) 

Symbiont research

The symbiont community of P. pelagicus was examined and compared. On 103 individuals in Khanh Hoa, we found 12 different species, 11 species were found on 75 individuals in Phu Quoc Island, and 5 species occur on 32 individuals in Phu Yen – Song Cau

The barnacle Octolasmis spp. is the most abundant ones, among that Octolasmis angulate was dominate species. These parasitic crab clearly showed to have negative impact on host populations. It is expected that the thermal gradient, from North to south, will be reflected in the symbiont community of the different crab populations. Our preliminary result has showed the trend of difference in species composition as well as geographically specific species.

The collected data will be used to establish the P. pelagicus/symbionts system as a general field indicator model for climate change in coastal waters.

We also start working on the population genetics of P. pelagicus using Next sequencing generation, as well as barnacle Octolasmis angulata using CO1 mitochndrial DNA.

Education and Conference presentation

Based on the recently obtained results, our students have presented at

The Young Conference on Biotechnology and Environment held in Nha Trang University, 16 May, 2016. The title as follow:

1)     Preleminary results on population genetics of barnacle (Octolasmis angulata) infected swimming crab (Portunus pelagicus) at Khanh Hoa (Nghiên cứu bước đầu  về đa dạng di truyền quần thể giáp xác chân tơ (Octolasmis angulata) ký sinh trên ghẹ xanh (Portunus pelagicus) ở Khánh Hòa)

2)     Species diversity of barnacle species infected swimming crab (Portunus pelagicus) at Khanh Hoa  (Thành phần các loài giáp xác chân tơ (Octolasmis spp.) ký sinh trên ghẹ xanh (Portunus pelagicus) tại Khánh Hòa)

The International Conference on Conservation genetics on Mekong Delta Basin

3)     Symbiont diversity of swimming crab (Portunus pelagicus)
in different temperature regimes in Vietnam

II. ECONOMIC COMPONENT (PD NGUYEN KHANH NGOC)

Research topic: Marine biodiversity loss and climate change: economic evaluation and implications for adaptation strategies - evidence from Vietnam by Quach Thi Khanh Ngoc
–Expected papers:
Paper 1: Economic evaluation of marine biodiversity loss linked to climate change: evidence from Vietnam.
Paper 2: Adaptation strategies for climate change impact on marine biodiversity , the case of Vietnam.

In 2017, Dr. Quach Thi Khanh Ngoc participated in different conferences in Singapore, Indonesia and Australia that helped her to present her works. In August, she presented her work funded by NORHED at the 7th Congress of the East Asian Association of Environmental and Resource Economics (EAAERE) “Environment and Sustainable Development: Issues, Challenges, and Solutions" in Singapore. She has gotten fresh perspectives on the research related to natural resources and climate change from this Congress.

            In November, she was invited to present her work on the climate change impact on coral reef ecosystem at the 3rd Monash Environmental Workshop in Australia. She has received valuable comments and suggestions for her paper from environmental economists from Australia, UK and US.

            Under the funding of UK research council, Ngoc also attended the workshop held in Indonesia: UK – Southeast Asia Researcher networking: Understanding the impact of hydrometeorological hazards in Southeast Asia. This workshop helps her to set up research network, and to share and to develop her research ideas.

            The funding of NORHED project bring her good opportunities for career development. She has one paper accepted in Marine Policy and has been revising another paper to resubmit to Ecosystem Services. 

III. AQUACULTURE COMPONENT (PD LE MINH HOANG)

Project title: Studies on effect of temperature and methionine in diet on juvenile cobia (Rachycentron canadum)

Project objective: The objective of this project is test to what extent elevated water temperatures increase the requirement for dietary methionine in juvenile cobia aquaculture.

Specific objective: The main objective of the present study is test to what extent elevated water temperatures increases the requirements for dietary methionine in cobia. The applied aspect is to generate the data that permits to maximise growth and N-retention in cobia. This will be done in two series of experiments with groups of cobia (juveniles) using different levels of dietary methionine and different temperature conditions.

Contents:

-          Effect of elevated water temperatures increase the requirement for dietary methionine on growth, body mass index, liver index, protein metabolism, lipid metabolism and feed efficiency cobia juveniles.

-          Effect of elevated water temperature increase the requirment for dietary methionine on feed intake, expression of NPY and CCK in juvenile cobia.

Methods: Details for methods have already presented in the approved research proposal.

Time schedule: This project included two experiments and on a survey of a status of cobia culture in the South, Central and North of Vietnam. The first experiment was carried out from May to October 2016. The second experiment also was carried out between March and May 2017. The survey for a status of cobia culture has been done in the North, Central of Vietnam and the South of Vietnam between August and October 2017.

Output:

-          01 Research proposal (done)

-          01 Annual report and final report (done)

-          At least 01 paper will participate in the international conference (done)

-          At least 01 paper will be published in national journal (02 draft manuscript)

-          At least 02 paper will be published in ISI journal (02 draft manuscript)

Activities have been done

  1. The research proposal was approved by supervisor and rector of Nha Trang University.
  2. The first experiment was carried out completely. All samples were analysed in Norway and used for writing 02 draft manuscript. The data of the first experiment were obtained and analysed at the end of December 2016 and used for the first of manuscript as draft and will be send to ISI journal from January to February 2017. The second experiment was carried out between March and May 2017. All samples of this experiment will be analysed in Norway from September to December 2017. The reamain samples will be analysed between April to June 2018. The second manuscript will be prepared and sent to ISI journal afterward. The tentative for sending the second paper will be between June and July 2018.

       3. Budget

1.1.  Scholarship used as following the research proposal (57,600NOK; 153,000,000 VND)

1.2.  Material and supply used as following the research proposal (205,608NOK; 546,900,000VND)

1.3.  Tool and equipment used as following the research proposal (320,370NOK; 852,159,269VND)

1.4.  Domestic travel used as following the research proposal (31,388NOK; 83,490,000VND)

1.5.  Data analysis used as following the research proposal (24,061NOK; 64,000,000VND)

1.6.  International travel will be transferred and used in 2018 (43,200NOK; 114,908,544VND).

4. Tentative Activities in 2018

  1. Manuscript preparing for submitting to ISI journal which used the data from the first experiment.
  1. The sample of second experiment will be analysed between September and December 2017. The remain samples will be analysed between April and June 2018 and data analysis will be performed afterward. These data will be prepared for the next manuscript that will be sent to ISI journal. Budget will be used from 2017 to transfer to 2018 (15,000 NOK for Travel expenses (air ticket round trip for PD students to Norway) and VISA fee 3,200 NOK). Total budget: 18,200 NOK (Budget from 2017 will be transferred to 2018).
  2. We are going to registration and participate to the appropriate international symposium. Budget from 2017 will be moved to 2018 (25,000 NOK).
  3. National paper will be prepared and sent to journal from February to April 2018.