PD Training

1. COMPONENT BIOTECHNOLOGY 

Project title: Effect of climate change on population dynamics of the swimming crab, Portunus pelagicus, and the epidemiology of selected disease causing organisms by PD Dang Thuy Binh

Recent obtained results

Sampling

crab, Portunus pelagicus was collected at Nha Trang Bay, O Loan Bay, and Song Cau (Khanh Hoa, Phu Yen, and Binh Dinh Province,) and Phu Quoc (Kien Giang Province) 

Symbiont research

The symbiont community of P. pelagicus was examined and compared. On 103 individuals in Khanh Hoa, we found 12 different species, 11 species were found on 75 individuals in Phu Quoc Island, and 5 species occur on 32 individuals in Phu Yen – Song Cau

The barnacle Octolasmis spp. is the most abundant ones, among that Octolasmis angulate was dominate species. These parasitic crab clearly showed to have negative impact on host populations. It is expected that the thermal gradient, from North to south, will be reflected in the symbiont community of the different crab populations. Our preliminary result has showed the trend of difference in species composition as well as geographically specific species.

The collected data will be used to establish the P. pelagicus/symbionts system as a general field indicator model for climate change in coastal waters.

We also start working on the population genetics of P. pelagicus using Next sequencing generation, as well as barnacle Octolasmis angulata using CO1 mitochndrial DNA.

Education and Conference presentation

Based on the recently obtained results, our students have presented at

The Young Conference on Biotechnology and Environment held in Nha Trang University, 16 May, 2016. The title as follow:

1)     Preleminary results on population genetics of barnacle (Octolasmis angulata) infected swimming crab (Portunus pelagicus) at Khanh Hoa (Nghiên cứu bước đầu  về đa dạng di truyền quần thể giáp xác chân tơ (Octolasmis angulata) ký sinh trên ghẹ xanh (Portunus pelagicus) ở Khánh Hòa)

2)     Species diversity of barnacle species infected swimming crab (Portunus pelagicus) at Khanh Hoa  (Thành phần các loài giáp xác chân tơ (Octolasmis spp.) ký sinh trên ghẹ xanh (Portunus pelagicus) tại Khánh Hòa)

The International Conference on Conservation genetics on Mekong Delta Basin

3)     Symbiont diversity of swimming crab (Portunus pelagicus)
in different temperature regimes in Vietnam

II. ECONOMIC COMPONENT

Research topic: Marine biodiversity loss and climate change: economic evaluation and implications for adaptation strategies - evidence from Vietnam by Quach Thi Khanh Ngoc
–Expected papers:
Paper 1: Economic evaluation of marine biodiversity loss linked to climate change: evidence from Vietnam.
Paper 2: Adaptation strategies for climate change impact on marine biodiversity , the case of Vietnam.

III. AQUACULTURE COMPONENT

Project title oEffects of altered temperature on aquaculture of cobia Rachycentron canadum: Optimizing the growth and N-retention- the role of methionine” by Le Minh Hoang

Norhed project was combined with Wisefeed project to determine the title, specific objective, main methods, contents and output of the project. The experiment will be conducted in the wetlab at Nha Trang University from 9th May 2016 to 2017.

The main objective of the present study is test to what extent elevated water temperatures increase the requirement for dietary methionine in cobia. The applied aspect is to generate the data that permits to maximise growth and N-retention in cobia. This will be done in two series of experiments with groups of cobia (juveniles) using different levels of dietary methionine and different temperature conditions.

Contents of the project:

-          Effect of temperature and methionine in diet on growth

-          Effect of temperature and methionine in diet on physiology and biochemistry

Main methods:

- Two series of experiments with groups of juvenile cobia and different temperature conditions (following by climate change) will be conducted. Three diets (low methionine, requirement methionine and surplus methionine) will be combined to two different temperatures (6 treatments) and repeated three times (18 experimental units). These experiments will be carried out for 6 weeks.

- The nutritional and molecular analysis will be computed and conducted at the beginning and the end of experiments by experts (NIFES – Norway, SPAROS – Portugal, CCMAR – Portugal, CSIC – Spain, UiB – Norway and NTU – Vietnam).

Expected Outputs:

-          01 paper will be participated in the international conference

-          01 paper will be published in national journal

-          01 paper will be published in ISI journal

Results:

1. Two experimental systems were designed at the wetlab in Nha Trang University: Experimental system for the room at 34ºC and Experimental system for the room at 30ºC.

2. Feed formulated diets which were produced at Portugal and transported to Nha Trang University: Three types of formulated diets (low methionine, required methionine, surplus methionine) were prepared and produced for a Wisefeed/Norhed trial with Cobia at SPAROS Lda – Portugal. These feeds (more than 100kg which size ranged from 1.6 mm to 2.0 mm) were produced from 02 March to 02 May 2016 and transported to Nha Trang University. They will be used for the experiment in May 2016 and the next year. (For more information: http://sparos.pt/index.php/en/news1/sparos-is-a-partner-in-h2020-project-wisefeed and http://wisefeed.b.uib.no/news/)

Experiments will be carried out at the wetlab in Nha Trang University:

- Juvenile cobia were bought and adapted for one week before experiment.

- The tentative time for experiment will be started on 9th May 2016 and performed for 6 months.

- All experiments will be done by experts (NIFES - Norway, SPAROS - Portugal, CCMAR - Portugal, CSIC - Spain, UiB - Norway and NTU - Vietnam).

- The results of these experiments (nutritional and molecular analysis) will later be conducted by experts in Nha Trang University or by staff from Nha Trang University to UiB and NIFES.

- All experiments in 2017 will be carried out similar to 2016 at the wetlab in Nha Trang University.